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eBook Kinesin Protocols (Methods in Molecular Biology) epub

by Isabelle Vernos

eBook Kinesin Protocols (Methods in Molecular Biology) epub
  • ISBN: 1617371785
  • Author: Isabelle Vernos
  • Genre: Medicine
  • Subcategory: Medicine
  • Language: English
  • Publisher: Humana Press; Softcover reprint of hardcover 1st ed. 2001 edition (November 10, 2010)
  • Pages: 258 pages
  • ePUB size: 1121 kb
  • FB2 size 1931 kb
  • Formats mobi mbr lrf txt


European Molecular Biology Laboratory, Heidelberg, Germany. Includes methods for determining ATPase activity, motility, and microtubule destabilizing activity.

European Molecular Biology Laboratory, Heidelberg, Germany. Details protocols to crystallize kinesin motor domains and study the structure of the kinesin-microtubule complex, and more. Download (pdf, . 5 Mb) Donate Read. Epub FB2 mobi txt RTF. Converted file can differ from the original. If possible, download the file in its original format.

In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible .

In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of t motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success

Methods in Molecular Biology.

Methods in Molecular Biology. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing.

Methods in molecular biology ; 212) Includes bibliographical references and index.

Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of t motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experim Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of t motors.

Methods in Molecular Biology™ is a trademark of The Humana Press Inc.

No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher.

By the end of the 1980s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified. At the time, these two motors seemed almost sufficient to explain directional motility events on polar microtubule tracks in the cell. No- theless, shortly after, the tip of the iceberg began to emerge with the identi- cation of proteins containing in their sequences a domain found in kinesin. This domain, called the “motor domain,” conferred on these proteins the essential property of moving on microtubules, using the energy derived from ATP hydro- sis. Since then, the identification of new proteins belonging to the kinesin superfamily of microtubule-dependent motors has gone at such a pace that nowadays more than 200 entries with motor domain sequences are deposited in the database. Kinesin family members are found in all eukaryotic org- isms tested. They present a wide range of domain organizations with a motor domain located at different positions in the molecule. Their motility prop- ties are also variable in directionality, velocity, and such other characteristics as bundling activity and processivity. Finally, and most important, they p- ticipate in a multitude of cellular functions. Our understanding of many cel- lar events, such as mitotic spindle assembly and neuronal transport, to cite only two, has progressed substantially in the last few years thanks to the id- tification of these motors.
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