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eBook Detection of Sigatoka Leaf Spot Pathogens of Banana by the Polymerase Chain Reaction epub

eBook Detection of Sigatoka Leaf Spot Pathogens of Banana by the Polymerase Chain Reaction epub
  • ISBN: 0859544753
  • Genre: No category
  • Language: English
  • Publisher: Hyperion Books
  • ePUB size: 1754 kb
  • FB2 size 1203 kb
  • Formats lrf lrf lit lrf


Detection Of Sigatoka.

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Published December 1997 by Hyperion Books. There's no description for this book yet.

The polymerase chain reaction products obtained with locus-specific primer pairs were digested with restriction enzymes to. .ABSTRACT Mycosphaerella musicola causes Sigatoka disease of banana and is endemic to Australia

The polymerase chain reaction products obtained with locus-specific primer pairs were digested with restriction enzymes to reveal polymorphism. Between five and 12 markers were polymorph in M. fijiensis populations from different geographical origins (Papua New Guinea, the Philippines, Cameroon and Latin America). ABSTRACT Mycosphaerella musicola causes Sigatoka disease of banana and is endemic to Australia. The population genetic structure of M. musicola in Australia was examined by applying single-copy restriction fragment length polymorphism probes to hierarchically sampled populations collected along the Australian east coast.

There are two Sigatoka leaf spot diseases of banana and plantain, yellow Sigatoka and black Sigatoka

There are two Sigatoka leaf spot diseases of banana and plantain, yellow Sigatoka and black Sigatoka. Yellow Sigatoka is caused by Mycosphaerella musicola, and black Sigatoka by M. fijiensis. Because unambiguous diagnosis of these leaf spots is often not possible by symptoms alone, we have used the polymerase chain reaction (PCR) to distinguish between the two species, and to detect the pathogens in DNA from infected leaf tissue. Two 21-base oligonucleotide primers were synthesised from a variable region identified in the nucleotide sequence of the internally transcribed spacer (ITS) 1 region.

Johanson, A. (1997) Detection of Sigatoka leaf spot of banana by polymerase chain reaction. Johanson, . & Jeger, M. J. (1993) Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spot in banana and plantain. Chatham, UK: National Resources Institute, University of Greenwich. 25. Mourichon, . Peter, . & Zapater, M. F. (1987) Experimental inoculation of young in␣vitro culture banana plantlets with Mycosphaerella fijiensis. CrossRefGoogle Scholar.

Black sigatoka (black leaf streak) caused by the fungus Mycosphaerella fijiensis, and yellow sigatoka caused by.

Black sigatoka (black leaf streak) caused by the fungus Mycosphaerella fijiensis, and yellow sigatoka caused by M. musicola, are common foliar pathogens of Musa species. Both species are pathogenic on East African Highland bananas (Musa spp. AAA) and may occur together on the same plant. A comprehensive diagnostic survey in Uganda in 1992 confirmed the presence of sigatoka leaf spots at most of the survey sites, but in most cases the species involved could not be determined due to the similarity of the symptoms they produce.

Black Sigatoka is a disease that occurs in banana plantations worldwide. No matrix effects were observed during the measurements of real leaf banana extracts by the immunosensor. This disease is caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results in a significant reduction in both product quality and yield. Therefore, detection and identification in the early stages of this pathogen in plants could help minimize losses, as well as prevent the spread of the disease to neighboring cultures. To achieve this, a highly sensitive SPR immunosensor was developed to detect P. fijiensis in real samples of leaf extracts in early stages of the disease.

Black Sigatoka is a leaf-spot disease of banana plants caused by the ascomycete fungus Mycosphaerella fijiensis (Morelet). Also known as black leaf streak, it was discovered in 1963 and named for its similarities with yellow Sigatoka, which is caused by Mycosphaerella musicola (Mulder), which was itself named after the Sigatoka Valley in Fiji, where an outbreak of this disease reached epidemic proportions from 1912 to 1923.

Black Sigatoka, also known as black leaf streak, is caused by Mycosphaerella fijiensis Morelet (anamorph Pseudocercospora . Detection of Sigatoka leaf spot pathogens of banana by the polymerase chain reaction. Chatman, UK, Natural Resource Institute, 1997.

Black Sigatoka, also known as black leaf streak, is caused by Mycosphaerella fijiensis Morelet (anamorph Pseudocercospora fijiensis (Morelet) Deighton). It is the most significant disease of bananas and plantains (Musa sp. because most of the economically important cultivars of exported and staple commodities are highly susceptible. The Caribbean is one of the few regions of the world where black Sigatoka is not widespread.

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