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This book provides an introduction to the use & applications of a field of growing importance in routine chemical analysis. It follows a logical progression from basic principles to instrumental and applications
This book provides an introduction to the use & applications of a field of growing importance in routine chemical analysis. It follows a logical progression from basic principles to instrumental and applications. This book is written for advanced undergraduates & immediate postgraduates in analytic chemistry, pharmaceutical analysis, applied biochemistry, biotechnology & pharmacology.
The basic principles of liquid chromatography and mass spectrometry. Written with a student audience in mind, this text will provide an invaluable introduction to this increasingly important analytical tandem technique. The limitations of the component techniques when used in isolation, and how a combination of the two allows these to be overcome. Descriptions of the various approaches, including thermospray, electrospray and atmospheric pressure chemical ionization, which are used to interface the two techniques, along with the advantages and disadvantages of each system.
WAINER Analysis and chromatography HPLC versus other analytical methods . 1 Volumetric analysis . 2 Ultraviolet spectrophotometry . 3 Using chromatography to improve specificity Historical development of chromatography HPLC today Bibliography Efficiency, retention, selectivity and resolution in chromatography . RILEY Introduction Chromatographic mobility Peak shape . Resolution (R,) Effect of peak asymmetry on column efficiency and separation Bibliography Modes of chromatography .
Liquid chromatography or high-pressure liquid (HPLC) is a popular quantitative analytical technique applied in many areas of chemical, biomedical and pharmaceutical sciences. HPLC is used for separation, detection and measurement of amino acids, proteins, lipids, carbohydrates, vitamins, trace elements and ions in various types of samples. It is also used for the analysis of polymorphisms in genes coding for specific proteins or enzymes.
High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography) is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.
Wainer, Irving W. Contents/Summary. High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. This book provides an introduction to the fundamentals of HPLC, written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines.
In I. W. Wainer (e., Liquid Chromatography in Pharmaceutical Development: An Introduction, Aster, Springfield, Or. 1985, pp 63–131. I. Wainer and M. C. Alembik. Xenobiotica 16:265–279 (1986). 11. A. Gulaid, G. Houghton, and A. R. Boobis.
Development and validation of liquid chromatography assays for the regulatory control of pharmaceuticals . The book is attractively hard bound.
Development and validation of liquid chromatography assays for the regulatory control of pharmaceuticals (. Comprehensive method validation strategy for bioanalytical applications in the pharmaceutical industry (.
The practitioners of modem liquid chromatography spend as much time in the development of techniques for sampling handling and automation as they do in the development of the separation.